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Applied Next-Generation Sequencing (NGS) & Genomic Analysis
Advanced

Applied Next-Generation Sequencing (NGS) & Genomic Analysis

Build and execute professional genomic pipelines for clinical and research applications.

5h 55mAdvanced
Instructor
Yasin Polat
01

Bioinformaticians building clinical analysis pipelines

02

Geneticists and molecular biologists analyzing NGS data

What You'll Learn

Design and execute end-to-end NGS bioinformatics pipelines
Perform rigorous quality control on raw sequencing data
Identify and annotate clinical germline variants using GATK best practices
Process and analyze RNA-Seq data for differential gene expression
Interpret complex VCF files for clinical or research reporting

About This Course

Master the complete bioinformatics pipeline from raw sequencing reads to clinical interpretation. This advanced course covers quality control, sequence alignment, and robust variant calling methodologies, with a special focus on germline analysis and transcriptomics.

Curriculum

9 ders · 2 ücretsiz önizleme

1
Introduction to sequencing technologies and FASTQ formatsÜcretsiz
25 dk
2
Read quality assessment with FastQC and MultiQCÜcretsiz
30 dk
3
Read trimming and adapter removal (Trimmomatic/Cutadapt)
35 dk
1
Read mapping to reference genomes (BWA-MEM & Bowtie2)
45 dk
2
SAM/BAM file processing and duplicate marking
35 dk
3
Germline variant calling pipelines with GATK HaplotypeCaller
55 dk
4
VCF annotation and clinical filtering strategies
40 dk
1
RNA-Seq alignment and read counting
40 dk
2
Differential expression analysis with DESeq2
50 dk

Requirements

  • Familiarity with Linux/Unix command line environment
  • Basic understanding of molecular genetics and sequencing
  • Prior experience with Python or R is highly recommended

Who This Is For

Bioinformaticians building clinical analysis pipelines
Geneticists and molecular biologists analyzing NGS data
Researchers working on whole-exome or whole-genome sequencing projects

Instructor

Yasin Polat

Bioinformatician & Researcher

Computational biologist specializing in clinical bioinformatics pipelines, germline variant analysis, and omics data integration.

Course Details

Level

Advanced

Total Duration

5h 55m

Course Type

Live Course

Language

Türkçe

Certificate

Included

Last Updated

2026-02

Frequently Asked Questions

Our platform offers both live and pre-recorded training; you can check the specific 'Course Type' at the top of the course page. Our live courses are fully interactive and conducted simultaneously with the instructor.

While many of our live sessions are recorded, this may vary depending on the specific course. If a course is recorded, you will have access to the recordings through your profile. Please check the course description for exact details.

You can ask your questions directly to the instructor during the live sessions. Additionally, you can stay in constant touch with the instructor and other participants through course-specific WhatsApp communication groups.

Yes, upon successful completion of the live training program, a personalized, verifiable certificate of completion is issued to you. You can add this certificate to your resume and LinkedIn profile with a single click.

Depending on the course, the installation of required software (Python, VS Code, etc.) is usually demonstrated step-by-step during the first session. For courses with specific hardware requirements, these are detailed in the 'Requirements' section on the course page.

To put the theoretical knowledge into practice, hands-on projects and assignments are given at the end of each week or module. You will receive instructor feedback by completing these assignments.

Free

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